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Vector Selection Guide

Vector Selection Guide

Find the right expression vector for your ORF clone by considering the expression host, promoter, bacterial selection, selection marker, copy number, or epitope tag. You could quick filter the resultes with your input into the second row of the table below.

For your convenience, you may be also interested to our Online Vector Builder Tool to construct the gene clones.

Vector Name Expression system Promoter Bacterial Selection Selection Maker Copy number Epitope tag Length(bp)
pET-3a Bacterial T7 Ampicillin medium N-T7 4640
pET-3b Bacterial T7 Ampicillin medium N-T7 4639
pET-3c Bacterial T7 Ampicillin medium N-T7 4638
pET-3d Bacterial T7 Ampicillin medium N-T7 4637
pET-9a Bacterial T7 kanamaycin medium N-T7 4341
pET-11a Bacterial T7 & Lac Ampicillin medium N-T7 5677
pET-11b Bacterial T7 & Lac Ampicillin medium N-T7 5676
pET-11c Bacterial T7 & Lac Ampicillin medium N-T7 5675
pET-11d Bacterial T7 & Lac Ampicillin medium N-T7 5674
pET-14b Bacterial T7 Ampicillin medium N-His 4671
pET-15b Bacterial T7 & Lac Ampicillin medium N-His 5708
pET-16b Bacterial T7 & Lac Ampicillin medium N-His 5711
pET-17b Bacterial T7 Ampicillin medium N-T7 3306
pET-19b Bacterial T7 & Lac Ampicillin medium N-His 5717
pET-20b(+) Bacterial T7 Ampicillin medium N-pelB; C-His 3716
pET-21a(+) Bacterial T7 & Lac Ampicillin medium N-T7; C-His 5443
pET-21b(+) Bacterial T7 & Lac Ampicillin medium N-T7; C-His 5442
pET-21d(+) Bacterial T7 & Lac Ampicillin medium N-T7; C-His 5440
pET-22b(+) Bacterial T7 & Lac Ampicillin medium N-pelB; C-His 5493
pET-23a(+) Bacterial T7 Ampicillin medium N-T7; C-His 3666
pET-24a(+) Bacterial T7 & Lac kanamaycin medium N-T7; C-His 5310
pET-24b(+) Bacterial T7 & Lac kanamaycin medium N-T7; C-His 5309
pET-24c(+) Bacterial T7 & Lac kanamaycin medium N-T7; C-His 5308
pET-24d(+) Bacterial T7 & Lac kanamaycin medium N-T7; C-His 5307
pET-25b(+) Bacterial T7 & Lac Ampicillin medium N-pelB; C-HSV, His 5547
pET-26b(+) Bacterial T7 & Lac kanamaycin medium N-pelB; C-His 5360
pET-27b(+) Bacterial T7 & Lac kanamaycin medium N-pelB;C-HSV, His 5414
pET-28a(+) Bacterial T7 & Lac kanamaycin medium N-His, T7; C-His 5369
pET-28b(+) Bacterial T7 & Lac kanamaycin medium N-His, T7; C-His 5368
pET-28c(+) Bacterial T7 & Lac kanamaycin medium N-His, T7; C-His 5367
pET-29a(+) Bacterial T7 & Lac kanamaycin medium N-S-tag; C-His 5371
pET-29b(+) Bacterial T7 & Lac kanamaycin medium N-S-tag; C-His 5370
pET-29c(+) Bacterial T7 & Lac kanamaycin medium N-S-tag; C-His 5372
pET-30a(+) Bacterial T7 & Lac kanamaycin medium N-His, S-tag; C-His 5422
pET-30b(+) Bacterial T7 & Lac kanamaycin medium N-His, S-tag; C-His 5421
pET-30c(+) Bacterial T7 & Lac kanamaycin medium N-His, S-tag; C-His 5423
pET-31b(+) Bacterial T7 & Lac Ampicillin medium N-KSI; C-His 5472
pET-32a(+) Bacterial T7 & Lac Ampicillin medium N-Trx; C-His 5900
pET-32b(+) Bacterial T7 & Lac Ampicillin medium N-Trx; C-His 5899
pET-41a(+) Bacterial T7 & Lac kanamaycin medium N-GST, His; C-S-tag, His 5933
pET-41b(+) Bacterial T7 & Lac kanamaycin medium N-GST, His; C-S-tag, His 5932
pET-41c(+) Bacterial T7 & Lac kanamaycin medium N-GST, His; C-S-tag, His 5934
pET-42a(+) Bacterial T7 & Lac kanamaycin medium N-GST, His; C-S-tag, His 5930
pET-42b(+) Bacterial T7 & Lac kanamaycin medium N-GST, His; C-S-tag, His 5929
pET-42c(+) Bacterial T7 & Lac kanamaycin medium N-GST, His; C-S-tag, His 5931
pET-43.1a(+) Bacterial T7 & Lac Ampicillin medium N-GST, His; C-S-tag, His 7275
pET-43.1b(+) Bacterial T7 & Lac Ampicillin medium N-GST, His; C-S-tag, His 7274
pET-45b(+) Bacterial T7 & Lac Ampicillin medium N-His; C-S-tag 5260
pET-50b(+) Bacterial T7 & Lac kanamaycin medium N-His, Nus; C-S-tag 6733
pET-51b(+) Bacterial T7 & Lac Ampicillin medium N-Strep; C-His 5218
pET-52b(+) Bacterial T7 & Lac Ampicillin medium N-Strep; C-His 5227
pGEX-2TK Bacterial Tac Ampicillin medium N-GST 4969
pGEX-4T-1 Bacterial Tac Ampicillin medium N-GST 4969
pGEX-4T-2 Bacterial Tac Ampicillin medium N-GST 4970
pGEX-4T-3 Bacterial Tac Ampicillin medium N-GST 4968
pGEX-5X-1 Bacterial Tac Ampicillin medium N-GST 4972
pGEX-5X-2 Bacterial Tac Ampicillin medium N-GST 4973
pGEX-5X-3 Bacterial Tac Ampicillin medium N-GST 4974
pGEX-6P-1 Bacterial Tac Ampicillin medium N-GST 4984
pGEX-6P-2 Bacterial Tac Ampicillin medium N-GST 4985
pGEX-6P-3 Bacterial Tac Ampicillin medium N-GST 4983
pMAL-c5E Bacterial Tac Ampicillin medium N-MBP 5677
pMAL-c5X Bacterial Tac Ampicillin medium N-MBP 6645
pMAL-p5E Bacterial Tac Ampicillin medium N-MBP 5755
pMAL-p5G Bacterial Tac Ampicillin medium N-MBP 5755
pMAL-p5X Bacterial Tac Ampicillin medium N-MBP 5752
pQE-60 Bacterial T5,lac Ampicillin medium N-His 3431
pGS-21a Bacterial T7,lac Ampicillin medium N-His, GST 6169
pETDuet-1 Bacterial T7,lac Ampicillin medium N-His 5420
pCDFDuet-1 Bacterial T7,lac Sm medium N-His 3781
pRSFDuet-1 Bacterial T7,lac kanamaycin medium N-His 3829
pCOLADuet-1 Bacterial T7,lac kanamaycin medium N-His 3719
pBAD/Myc-His A Bacterial araBad Ampicillin medium C-His, C-Myc 4094
pCold I Bacterial CSPA Ampicillin medium N-His, N-Factor Xa 4407
pCold II Bacterial CSPA Ampicillin medium N-His 4392
pCold IV Bacterial CSPA Ampicillin medium 4359
pcDNA3.1(+) Mammalian cmv Ampicillin High 5431
pcDNA3.1(-) Mammalian cmv Ampicillin High 5430
pcDNA3.1(+)_myc-His A Mammalian cmv Ampicillin High C-Myc, His 5491
pcDNA3.1(+)_myc-His B Mammalian cmv Ampicillin High C-Myc, His 5495
pcDNA3.1(+)_myc-His C Mammalian cmv Ampicillin High C-Myc, His 5487
pcDNA3.1(-)_myc-His A Mammalian cmv Ampicillin High C-Myc, His 5522
pcDNA3.1(-)_myc-His B Mammalian cmv Ampicillin High C-Myc, His 5520
pcDNA3.1(-)_myc-His C Mammalian cmv Ampicillin High C-Myc, His 5521
pcDNA3.1/Hygro(+) Mammalian cmv Ampicillin Hygromycin High 5600
pcDNA3.1/Hygro(-) Mammalian cmv Ampicillin Hygromycin High 5599
pcDNA3.1/Zeo (+) Mammalian cmv Ampicillin Zeocin High 5015
pcDNA3.1/Zeo (-) Mammalian cmv Ampicillin Zeocin High 5014
pcDNA3.1+C-eGFP Mammalian cmv Ampicillin High C-eGFP  6143
pcDNA3.1+N-eGFP Mammalian cmv Ampicillin High N-eGFP  6134
pcDNA3.1+N-DYK Mammalian cmv Ampicillin High N-DYKDDDDK 5453
pcDNA3.1+C-DYK Mammalian cmv Ampicillin High C-DYKDDDDK 5444
pcDNA3.1+N-6His Mammalian cmv Ampicillin High N-His 5441
pcDNA3.1+C-6His Mammalian cmv Ampicillin High C-HIS 5432
pcDNA3.1+N-HA Mammalian cmv Ampicillin High N-HA 5450
pcDNA3.1+C-HA Mammalian cmv Ampicillin High C-HA 5441
pcDNA3.1+N-Myc Mammalian cmv Ampicillin High N-Myc 5453
pcDNA3.1+C-Myc Mammalian cmv Ampicillin High C-MYC 5444
pEGFP-N1 Mammalian cmv Kanamycin Neomycin High C-eGFP  4733
pEGFP-N2 Mammalian cmv Kanamycin Neomycin High C-eGFP  4737
pEGFP-C1 Mammalian cmv Kanamycin Neomycin High N-EGFP 4731
pTT5 Mammalian cmv Ampicillin High 4401
pIRES2-EGFP Mammalian cmv Kanamycin Neomycin High C-EGFP 5308
pCEP4 Mammalian cmv Ampicillin Hygromycin High 10186
pFastBac 1 Baculovirus polyhedrin Ampicillin high 4776
pFastBac HT-A Baculovirus polyhedrin Ampicillin high N-His,TEV 4856
pFastBac HT-B Baculovirus polyhedrin Ampicillin high N-His,TEV 4857
pFastBac HT-C Baculovirus polyhedrin Ampicillin high N-His,TEV 4858
pFastBac-Dual Baculovirus polyhedrin, p10 Ampicillin high 5238
pAO815 Pichia AOX1 Ampicillin high 7700
pPIC 3.5k Pichia AOX1 Ampicillin high 9004
pPIC9 Pichia AOX1 Ampicillin high N-伪-factor secretion signal 8023
pPIC9K Pichia AOX1 Ampicillin high 9276
pPICZ A Pichia AOX1 Zeocin high C-Myc, His 3329
pPICZ B Pichia AOX1 Zeocin high C-Myc, His 3328
pPICZ C Pichia AOX1 Zeocin high C-Myc, His 3329
pPICZα A Pichia AOX1 Zeocin high N-α-factor secretion signal; C-Myc, His 3593
pPICZα B Pichia AOX1 Zeocin high N-α-factor secretion signal; C-Myc, His 3597
pPICZα C Pichia AOX1 Zeocin high N-α-factor secretion signal; C-Myc, His 3597
pESC-TRP S. cerevisiae GAL1, GAL10 Ampicillin high C-DYKDDDDK, Myc 6530
pESC-URA S. cerevisiae GAL1, GAL10 Ampicillin high C-DYKDDDDK, Myc 6631
pESC-HIS S. cerevisiae GAL1, GAL10 Ampicillin high C-DYKDDDDK, Myc 6710
pESC-LEU S. cerevisiae GAL1, GAL10 Ampicillin high C-DYKDDDDK, Myc 7746

Souce: NovoPro    2018-05-04