Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V010239 | pPICZα C | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
pPICZα C is a pichia pastoris vector for methanol-inducible intracellular expression of a protein. For other reading frames, use pPICZα B or pPICZα C.
pPICZα C vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Li JX, Zhao LM, Wu RJ, Zheng ZJ, Zhang RJ. High-level overproduction of Thermobifida enzyme in Streptomyces lividans using a novel expression vector. Int J Mol Sci. 2013 Sep 10;14(9):18629-39.
pPICZα C vector Sequence
LOCUS pPICZ-C. 3598 bp DNA circular SYN 13-SEP-2021
DEFINITION Pichia pastoris vector for methanol-inducible expression of a
secreted protein.
ACCESSION .
VERSION .
KEYWORDS pPICZ-C.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3598)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 3598)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 3598)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT SGRef: number: 2; type: "Journal Article"
COMMENT Linearize with SacI, PmeI, or BstXI for integration at the AOX1
locus.
FEATURES Location/Qualifiers
source 1..3598
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 2..940
/label=AOX1 promoter
/note="inducible promoter, regulated by methanol"
CDS 941..1207
/label=alpha-factor secretion signal
/note="N-terminal secretion signal from S. cerevisiae
alpha-factor"
misc_feature 1207..1281
/label=MCS
/note="MCS"
/note="MCS;multiple cloning site"
CDS 1280..1309
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
CDS 1325..1342
/label=6xHis
/note="6xHis affinity tag"
terminator 1422..1668
/label=AOX1 terminator
/note="transcription terminator for AOX1"
promoter 1683..2094
/label=TEF1 promoter
/note="promoter for EF-1-alpha"
promoter 2102..2149
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 2168..2539
/label=BleoR
/note="antibiotic-binding protein"
terminator 2608..2855
/label=CYC1 terminator
/note="transcription terminator for CYC1"
rep_origin complement(2930..3518)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"