Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V011006 | pET-27b(+) | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
pET-27b(+) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pET-27b(+) vector Sequence
LOCUS pET-27b(+). 5414 bp DNA circular SYN 01-JAN-1980
DEFINITION Bacterial vector with a signal sequence and a kanamycin resistance
marker for expression of C-terminally HSV-tagged proteins in the
periplasm.
ACCESSION .
VERSION .
KEYWORDS pET-27b(+).
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5414)
AUTHORS Novagen (EMD Millipore)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5414)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5414
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(26..73)
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(140..157)
/label=6xHis
/note="6xHis affinity tag"
CDS complement(164..196)
/label=HSV tag
/note="HSV (herpes simplex virus) epitope tag"
misc_feature 212..279
/label=MCS
/note="MCS"
/note="multiple cloning site"
sig_peptide complement(278..343)
/label=pelB signal sequence
/note="leader peptide for secretion"
RBS complement(351..373)
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
protein_bind complement(388..412)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(413..431)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 740..817
/label=lacI promoter
CDS 818..1897
/label=lacI
/note="lac repressor"
protein_bind 1913..1934
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS 2709..2897
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
misc_feature 3002..3144
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(3330..3918)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 4040..4852
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin complement(4948..5403)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"