pEGFP-C1 vector (V012024)

Price Information

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

• • • Vector Name:
    • pEGFP-C1
  • • Antibiotic Resistance:
    • Kanamycin
• • • Length:
    • 4731 bp
  • • Type:
    • Fluorescent Protein Genes & Plasmids
• • • Replication origin:
    • ori
  • • Source/Author:
    • Clontech
• • • Selection Marker:
    • Neomycin/G418(Geneticin)
  • • Copy Number:
    • High copy number
• • • Promoter:
    • CMV
  • • Cloning Method:
    • Enzyme Cut
• • • Growth Strain(s):
    • JM108
  • • Growth Temperature:
    • 37℃
• • • Expression Method:
    • Transient

pEGFP-C1 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

• Payán-Bravo L, Fontalva S, Peñate X, Cases I, Guerrero-Martínez JA, Pareja-Sánchez Y, Odriozola-Gil Y, Lara E, Jimeno-González S, Suñé C, Muñoz-Centeno MC, Reyes JC, Chávez S. Human prefoldin modulates co-transcriptional pre-mRNA splicing. Nucleic Acids Res. 2021 Jun 21;49(11):6267-6280.  doi: 10.1093/nar/gkab446
• Payán-Bravo L, Fontalva S, Peñate X, Cases I, Guerrero-Martínez JA, Pareja-Sánchez Y, Odriozola-Gil Y, Lara E, Jimeno-González S, Suñé C, Muñoz-Centeno MC, Reyes JC, Chávez S. Human prefoldin modulates co-transcriptional pre-mRNA splicing. Nucleic Acids Res. 2021 Jun 21;49(11):6267-6280. doi: 10.1093/nar/gkab446
• Medlej A, Mohammad Soltani B, Javad Mowla S, Hosseini S, Baharvand H. A novel miRNA located in the GATA4 gene regulates the expression of IGF-1R and AKT1/2 genes and controls cell proliferation. J Cell Biochem. 2020;121(5-6):3438-3450. doi:10.1002/jcb.29617
• Asselin L, Rivera Alvarez J, Heide S, et al. Mutations in the KIF21B kinesin gene cause neurodevelopmental disorders through imbalanced canonical motor activity. Nat Commun. 2020;11(1):2441. doi:10.1038/s41467-020-16294-6
• Kim HJ, Kim HJ, Kim MK, et al. SPSB1 enhances ovarian cancer cell survival by destabilizing p21. Biochem Biophys Res Commun. 2019;510(3):364-369 https://doi.org/10.1016/j.bbrc.2019.01.088
• Namyanja,   Monica & Xu, Zhi-Shen & Mugasa, Claire & Lun, Zhao-Rong &   Matovu, Enock & Chen, Zhengjun & Lubega, George. (2019). Preliminary   evaluation of a Trypanosoma brucei FG-GAP repeat containing protein of   mitochondrial localization. AAS Open Research. 2. 165. 10.12688/aasopenres.12986.1.  https://aasopenresearch.org/articles/2-165
• Abildgaard   AB, Stein A, Nielsen SV, et al. Computational and cellular studies reveal structural destabilization and degradation of MLH1 variants in Lynch   syndrome. Elife. 2019 https://doi.org/10.7554/eLife.49138

pEGFP-C1 vector Sequence

LOCUS       pEGFP-C1.        4731 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for fusing EGFP to the N-terminus of a partner protein. For 
            other reading frames, use pEGFP-C2 or pEGFP-C3.
ACCESSION   .
VERSION     .
KEYWORDS    pEGFP-C1.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    1330..1395
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    1404..1414
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    1519..1640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1647..2102)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2129..2233
                     /label=AmpR promoter
     promoter        2235..2592
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2627..3418
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3653..3700
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4029..4617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"