Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V011035 | pCOLADuet-1 | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
pCOLADuet-1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pCOLADuet-1 vector Sequence
LOCUS pCOLADuet-1. 3719 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector with a ColA origin for the co-expression of two genes. ACCESSION . VERSION . KEYWORDS pCOLADuet-1. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3719) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 3719) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3719 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 3..27 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 42..64 /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 71..73 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 83..100 /label=6xHis /note="6xHis affinity tag" promoter 214..232 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 233..257 /label=lac repressor encoded by lacI binding site /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 286..291 /note="ribosome binding site" CDS 300..302 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 366..410 /label=S-Tag /note="affinity and epitope tag derived from pancreatic ribonuclease A" terminator 462..509 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(742..1554) /label=KanR /note="aminoglycoside phosphotransferase" promoter complement(1555..1646) /label=AmpR promoter rep_origin complement(1664..2299) /direction=LEFT /label=ColA ori /note="Plasmids containing the ColA origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins." protein_bind complement(2463..2484) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS complement(2500..3579) /label=lacI /note="lac repressor" promoter complement(3580..3657) /label=lacI promoter