Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V010975 | pET-41a(+) | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
- Vector Name:
- pET-41a(+)
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5933 bp
- Type:
- pET & Duet Vectors (Novagen)
- Replication origin:
- ori
- Source/Author:
- Novagen (EMD Millipore)
- Copy Number:
- High copy number
- Growth Strain(s):
- DH10B
- Growth Temperature:
- 37℃
pET-41a(+) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pET-41a(+) vector Sequence
LOCUS pET-41a(+). 5933 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for expressing GST fusion proteins with an enterokinase site. For other reading frames, use pET-41b(+) or pET-41c(+). ACCESSION . VERSION . KEYWORDS pET-41a(+). SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5933) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 5933) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5933 /mol_type="other DNA" /organism="synthetic DNA construct" terminator complement(26..73) /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(150..173) /label=8xHis /note="8xHis affinity tag" misc_feature 174..265 /label=MCS /note="MCS" /note="multiple cloning site" CDS complement(265..279) /label=enterokinase site /note="enterokinase recognition and cleavage site" CDS complement(310..354) /label=S-Tag /note="affinity and epitope tag derived from pancreatic ribonuclease A" CDS complement(370..387) /label=thrombin site /note="thrombin recognition and cleavage site" CDS complement(397..414) /label=6xHis /note="6xHis affinity tag" CDS complement(442..1095) /label=GST /note="glutathione S-transferase from Schistosoma japonicum" RBS complement(1103..1125) /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(1140..1164) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1165..1183) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 1496..1573 /label=lacI promoter CDS 1574..2653 /label=lacI /note="lac repressor" protein_bind 2669..2690 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS 3228..3416 /label=rop /note="Rop protein, which maintains plasmids at low copy number" misc_feature 3521..3663 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(3849..4437) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS 4559..5371 /label=KanR /note="aminoglycoside phosphotransferase" rep_origin complement(5467..5922) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"