pET-14b vector (V011030)

MSDS

Basic Vector Information

- - Vector Name:
  - pET-14b
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 4671 bp
- - Type:
  - pET & Duet Vectors (Novagen)
- - Replication origin:
  - ori
- - Source/Author:
  - Novagen (EMD Millipore)
- - Copy Number:
  - High copy number
- - Promoter:
  - tet

pET-14b vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pET-14b vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pET-14b.        4671 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expression of N-terminally 6xHis-tagged 
            proteins.
ACCESSION   .
VERSION     .
KEYWORDS    pET-14b.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     terminator      complement(404..451)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    510..526
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             complement(527..544)
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             complement(554..571)
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             complement(581..583)
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     RBS             complement(590..612)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(644..662)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2223..2411
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2516..2658
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2844..3432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3606..4463)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4464..4568)
                     /label=AmpR promoter

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