Basic Vector Information
- Vector Name:
- pGEX-6P-2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4985 bp
- Type:
- pGEX Vectors (GE Healthcare)
- Replication origin:
- ori
- Source/Author:
- GE Healthcare
- Copy Number:
- High copy number
pGEX-6P-2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pGEX-6P-2 vector Sequence
LOCUS pGEX-6P-2. 4985 bp DNA circular SYN 01-JAN-1980
DEFINITION Bacterial vector for expressing GST fusion proteins with a
PreScission protease site. For other reading frames, use pGEX-6P-1
or pGEX-6P-3.
ACCESSION .
VERSION .
KEYWORDS pGEX-6P-2.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4985)
AUTHORS GE Healthcare
TITLE Direct Submission
REFERENCE 2 (bases 1 to 4985)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4985
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 183..211
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 258..911
/label=GST
/note="glutathione S-transferase from Schistosoma
japonicum"
CDS 918..941
/label=HRV 3C site
/note="recognition and cleavage site for human rhinovirus
3C and PreScission proteases"
misc_feature 940..982
/label=MCS
/note="MCS"
/note="multiple cloning site"
misc_feature 987..997
/label=stop codons
/note="stop codons"
/note="stop codons in all three reading frames"
promoter 1288..1392
/label=AmpR promoter
CDS 1393..2250
/label=AmpR
/note="beta-lactamase"
rep_origin 2424..3012
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 3256..3333
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 3334..4413
/label=lacI
/note="lac repressor"
protein_bind 4429..4450
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4465..4495
/label=lac promoter
/note="promoter for the E. coli lac operon"
CDS 4539..4712
/label=lacZ-alpha
/note="LacZ-alpha fragment of beta-galactosidase"
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