Basic Vector Information
- Vector Name:
- pcDNA3.1+/C-DYK
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5438 bp
- Type:
- Mammalian Expression Vectors
- Replication origin:
- ori
- Source/Author:
- GenScript
- Selection Marker:
- Neomycin/G418(Geneticin)
- Copy Number:
- High copy number
- Promoter:
- lac
pcDNA3.1+/C-DYK vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pcDNA3.1+/C-DYK vector Sequence
LOCUS pcDNA3.1+_C-DYK. 5438 bp DNA circular SYN 01-JAN-1980
DEFINITION Mammalian expression vector with a CMV promoter for expressing
proteins with a C-terminal FLAG(R) (DYK) tag.
ACCESSION .
VERSION .
KEYWORDS pcDNA3.1+ C-DYK.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5438)
AUTHORS GenScript
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5438)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5438
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 895..985
/label=MCS
/note="MCS"
/note="multiple cloning site"
CDS 986..1009
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
polyA_signal 1035..1259
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1305..1733
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1747..2076
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 2143..2934
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 3111..3244
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3281..3297)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3305..3321)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3329..3359)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3374..3395)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3683..4271)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4445..5302)
/label=AmpR
/note="beta-lactamase"
promoter complement(5303..5407)
/label=AmpR promoter
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