Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V011647 | pFastBacHT A | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
- Vector Name:
- pFastBacHT A
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4856 bp
- Type:
- Insect Cell Vectors
- Replication origin:
- ori
- Source/Author:
- Invitrogen (Life Technologies)
- Copy Number:
- High copy number
- Promoter:
- polyhedrin
pFastBacHT A vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pFastBacHT A vector Sequence
LOCUS pFastBacHT_A. 4856 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for expressing 6xHis-TEV-tagged proteins in insect cells using the Bac-to-Bac(R) baculovirus system. For other reading frames, use pFastBac(TM)HT B or pFastBac(TM)HT C. ACCESSION . VERSION . KEYWORDS pFastBacHT A. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4856) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 4856) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT Transform into DH10Bac(TM) E. coli cells. FEATURES Location/Qualifiers source 1..4856 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 2..457 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 484..588 /label=AmpR promoter CDS 589..1446 /label=AmpR /note="beta-lactamase" rep_origin 1620..2208 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" mobile_element complement(2511..2735) /label=Tn7R /note="mini-Tn7 element (right end of the Tn7 transposon)" CDS complement(2805..3335) /label=GmR /note="gentamycin acetyltransferase" promoter complement(3524..3552) /label=Pc promoter /note="class 1 integron promoter" promoter 3904..3995 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" CDS 4050..4052 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 4062..4079 /label=6xHis /note="6xHis affinity tag" CDS 4101..4121 /label=TEV site /note="tobacco etch virus (TEV) protease recognition and cleavage site" misc_feature 4119..4222 /label=MCS /note="MCS" /note="multiple cloning site" polyA_signal 4346..4480 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" mobile_element complement(4509..4674) /label=Tn7L /note="mini-Tn7 element (left end of the Tn7 transposon)"