pPICZα B vector (V010240)

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V010240	pPICZα B	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

pPICZα B vector (V010240) is a Pichia pastoris vector for methanol-inducible expression of a secreted protein. There are other similar expression vectors, such as pPICZα A or pPICZα C For other reading frames.

- - Vector Name:
  - pPICZα B
- - Antibiotic Resistance:
  - Bleomycin
- - Length:
  - 3597 bp
- - Type:
  - Yeast Plasmids
- - Replication origin:
  - ori
- - Source/Author:
  - Invitrogen (Life Technologies)
- - Copy Number:
  - High copy number
- - Promoter:
  - AOX1
- - Growth Strain(s):
  - Stbl3
- - Growth Temperature:
  - 37℃

pPICZα B vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

Zhao RL, Zhang XM, Jia LN, Song W, Sun YL, Meng XY, Peng XX. pNNS-Conjugated Chitosan Mediated IGF-1 and miR-140 Overexpression in Articular Chondrocytes Improves Cartilage Repair. Biomed Res Int. 2019 Mar 21;2019:2761241.

pPICZα B vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pPICZ-B.        3597 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Pichia pastoris vector for methanol-inducible expression of a 
            secreted protein.
ACCESSION   .
VERSION     .
KEYWORDS    pPICZ-B.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3597)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3597)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Linearize with SacI, PmeI, or BstXI for integration at the AOX1 
            locus.
FEATURES             Location/Qualifiers
     source          1..3597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1207
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     misc_feature    1206..1280
                     /label=MCS
                     /note="MCS"
                     /note="MCS;multiple cloning site"
     CDS             1279..1308
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1324..1341
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      1421..1667
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1682..2093
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2101..2148
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2167..2538
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      2607..2854
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2929..3517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"