pFastBacHT B vector (V011646)

MSDS

Basic Vector Information

- - Vector Name:
  - pFastBacHT B
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 4857 bp
- - Type:
  - Insect Cell Vectors
- - Replication origin:
  - ori
- - Source/Author:
  - Invitrogen (Life Technologies)
- - Copy Number:
  - High copy number
- - Promoter:
  - polyhedrin

pFastBacHT B vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pFastBacHT B vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pFastBacHT_B.        4857 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing 6xHis-TEV-tagged proteins in insect cells 
            using the Bac-to-Bac(R) baculovirus system. For other reading 
            frames, use pFastBac(TM)HT A or pFastBac(TM)HT C.
ACCESSION   .
VERSION     .
KEYWORDS    pFastBacHT B.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Transform into DH10Bac(TM) E. coli cells.
FEATURES             Location/Qualifiers
     source          1..4857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /label=AmpR promoter
     CDS             589..1446
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1620..2208
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(2511..2735)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2805..3335)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(3524..3552)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4050..4052
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             4062..4079
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             4101..4121
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    4119..4223
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    4347..4481
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(4510..4675)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"

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