Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V010241 | pPICZα A | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
The pPICZα A is a Pichia pastoris vector for methanol-inducible expression of a secreted protein.
pPICZα A vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Dong C, Xu L, Lu W, Li M, Zhang R, Sun Y, Liu J, Chu X. Antibacterial peptide PMAP-37(F34-R), expressed in Pichia pastoris, is effective against pathogenic bacteria and preserves plums. Microb Cell Fact. 2023 Aug 27;22(1):164.
pPICZα A vector Sequence
LOCUS pPICZ-A. 3593 bp DNA circular SYN 13-SEP-2021 DEFINITION Pichia pastoris vector for methanol-inducible expression of a secreted protein. ACCESSION . VERSION . KEYWORDS pPICZ-A. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3593) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 3593) TITLE Direct Submission REFERENCE 3 (bases 1 to 3593) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT SGRef: number: 2; type: "Journal Article" COMMENT Linearize with SacI, PmeI, or BstXI for integration at the AOX1 locus. FEATURES Location/Qualifiers source 1..3593 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 2..940 /label=AOX1 promoter /note="inducible promoter, regulated by methanol" CDS 941..1207 /label=alpha-factor secretion signal /note="N-terminal secretion signal from S. cerevisiae alpha-factor" misc_feature 1208..1276 /label=MCS /note="MCS" /note="MCS;multiple cloning site" CDS 1275..1304 /label=Myc /note="Myc (human c-Myc proto-oncogene) epitope tag" CDS 1320..1337 /label=6xHis /note="6xHis affinity tag" terminator 1417..1663 /label=AOX1 terminator /note="transcription terminator for AOX1" promoter 1678..2089 /label=TEF1 promoter /note="promoter for EF-1-alpha" promoter 2097..2144 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 2163..2534 /label=BleoR /note="antibiotic-binding protein" terminator 2603..2850 /label=CYC1 terminator /note="transcription terminator for CYC1" rep_origin complement(2925..3513) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"