pRSFDuet-1 vector (V010923)

Price Information

Cat No. Plasmid Name Availability Add to cart
V010923 pRSFDuet-1 In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

The pRSFDuet-1 is a bacterial vector with an RSF 1030 origin for the co-expression of two genes.

      • Vector Name:
      • pRSFDuet-1
      • Antibiotic Resistance:
      • Kanamycin
      • Length:
      • 3829 bp
      • Type:
      • pET & Duet Vectors (Novagen)
      • Source/Author:
      • Novagen (EMD Millipore)
      • Copy Number:
      • High copy number
      • Growth Strain(s):
      • JM108
      • Growth Temperature:
      • 37℃

pRSFDuet-1 vector Vector Map

pRSFDuet-13829 bp60012001800240030003600lac operatorRBSATG6xHisT7 promoterlac operatorRBSATGS-TagT7 terminatorKanRAmpR promoterRSF oriCAP binding sitelacIlacI promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

  • Chen Y, Ma B, Cao S, Wu X, Xu Y. Efficient synthesis of Ibrutinib chiral intermediate in high space-time yield by recombinant E. coli co-expressing alcohol dehydrogenase and glucose dehydrogenase. RSC Adv. 2019 Jan 18;9(4):2325-2331. doi: 10.1039/c8ra08100j. PMID: 35516114; PMCID: PMC9059822.

pRSFDuet-1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pRSFDuet-1.        3829 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector with an RSF 1030 origin for the co-expression of 
            two genes.
ACCESSION   .
VERSION     .
KEYWORDS    pRSFDuet-1.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             71..73
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             83..100
                     /label=6xHis
                     /note="6xHis affinity tag"
     promoter        214..232
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    233..257
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             286..291
                     /note="ribosome binding site"
     CDS             300..302
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             366..410
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     terminator      462..509
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(742..1554)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(1555..1646)
                     /label=AmpR promoter
     rep_origin      complement(1662..2411)
                     /direction=LEFT
                     /label=RSF ori
                     /note="Plasmids containing the RSF 1030 origin of
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     protein_bind    complement(2573..2594)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2610..3689)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(3690..3767)
                     /label=lacI promoter