Basic Vector Information
- Vector Name:
- pET-23a(+)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3666 bp
- Type:
- pET & Duet Vectors (Novagen)
- Replication origin:
- ori
- Source/Author:
- Novagen (EMD Millipore)
- Copy Number:
- High copy number
- Promoter:
- T7
pET-23a(+) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pET-23a(+) vector Sequence
LOCUS pET-23a(+). 3666 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for expression of N-terminally T7-tagged proteins. For other reading frames, use pET-23b(+) or pET-23c(+). ACCESSION . VERSION . KEYWORDS pET-23a(+). SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3666) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 3666) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3666 /mol_type="other DNA" /organism="synthetic DNA construct" terminator complement(26..73) /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(140..157) /label=6xHis /note="6xHis affinity tag" misc_feature 158..203 /label=MCS /note="MCS" /note="multiple cloning site" CDS complement(207..239) /label=T7 tag (gene 10 leader) /note="leader peptide from bacteriophage T7 gene 10" RBS complement(247..269) /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" promoter complement(301..319) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 828..1016 /label=rop /note="Rop protein, which maintains plasmids at low copy number" misc_feature 1121..1263 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(1449..2037) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2211..3068) /label=AmpR /note="beta-lactamase" promoter complement(3069..3173) /label=AmpR promoter rep_origin complement(3200..3655) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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