Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V010930 | pETDuet-1 | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
- Vector Name:
- pETDuet-1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5420 bp
- Type:
- pET & Duet Vectors (Novagen)
- Replication origin:
- ori
- Source/Author:
- Novagen (EMD Millipore)
- Copy Number:
- High copy number
pETDuet-1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pETDuet-1 vector Sequence
LOCUS pETDuet-1. 5420 bp DNA circular SYN 01-JAN-1980
DEFINITION Bacterial vector for the co-expression of two genes.
ACCESSION .
VERSION .
KEYWORDS pETDuet-1.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5420)
AUTHORS Novagen (EMD Millipore)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5420)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5420
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 3..27
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 42..64
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 71..73
/codon_start=1
/product="start codon"
/label=start codon
/note="ATG"
/translation="M"
CDS 83..100
/label=6xHis
/note="6xHis affinity tag"
promoter 214..232
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 233..257
/label=lac repressor encoded by lacI binding site
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 286..291
/note="ribosome binding site"
CDS 300..302
/codon_start=1
/product="start codon"
/label=start codon
/note="ATG"
/translation="M"
CDS 366..410
/label=S-Tag
/note="affinity and epitope tag derived from pancreatic
ribonuclease A"
terminator 462..509
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 546..1001
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(1119..1976)
/label=AmpR
/note="beta-lactamase"
promoter complement(1977..2069)
/label=AmpR promoter
rep_origin 2150..2738
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(2924..3066)
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(3171..3359)
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
protein_bind complement(3897..3918)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(3934..5013)
/label=lacI
/note="lac repressor"
promoter complement(5014..5091)
/label=lacI promoter