Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V010320 | pESC-TRP | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
The pESC-TRP is a yeast episomal vector with a TRP1 marker, for galactose-regulated expression and tagging of up to two genes.
pESC-TRP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Yu KO, Jung J, Ramzi AB, Choe SH, Kim SW, Park C, Han SO. Development of a Saccharomyces cerevisiae strain for increasing the accumulation of triacylglycerol as a microbial oil feedstock for biodiesel production using glycerol as a substrate. Biotechnol Bioeng. 2013 Jan;110(1):343-7. doi: 10.1002/bit.24623.
- Honjoh K, Matsuura K, Machida T, Nishi K, Nakao M, Yano T, Miyamoto T, Iio M. Enhancement of menadione stress tolerance in yeast by accumulation of hypotaurine and taurine: co-expression of cDNA clones, from Cyprinus carpio, for cysteine dioxygenase and cysteine sulfinate decarboxylase in Saccharomyces cerevisiae. Amino Acids. 2010 Apr;38(4):1173-83.
pESC-TRP vector Sequence
LOCUS 40924_17611 6525 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pESC-TRP, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6525) AUTHORS Lu Q. TITLE pESC-TRP JOURNAL Unpublished REFERENCE 2 (bases 1 to 6525) AUTHORS Lu Q. TITLE Direct Submission JOURNAL Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines Rd., La Jolla, CA 92037, USA REFERENCE 3 (bases 1 to 6525) TITLE Direct Submission REFERENCE 4 (bases 1 to 6525) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines Rd., La Jolla, CA 92037, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6525 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 187..467 /label=TRP1 promoter CDS 468..1139 /codon_start=1 /label=TRP1 /note="phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA KK" rep_origin complement(1241..1696) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" terminator complement(1779..1944) /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" CDS complement(2092..2115) /codon_start=1 /label=FLAG /note="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /translation="DYKDDDDK" promoter 2162..2826 /label=GAL1,10 promoter /note="divergent inducible promoter, regulated by Gal4" promoter 2837..2855 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 2873..2902 /codon_start=1 /label=Myc /note="Myc (human c-Myc proto-oncogene) epitope tag" /translation="EQKLISEEDL" terminator 2932..3121 /label=CYC1 terminator /note="transcription terminator for CYC1" rep_origin complement(3364..3952) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4126..4983) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4984..5088) /label=AmpR promoter rep_origin complement(5115..6457) /direction=LEFT /label=2u ori /note="yeast 2u plasmid origin of replication"