pET-20b(+) vector (V011025)

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V011025	pET-20b(+)	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

Vector Name:: pET-20b(+)

Antibiotic Resistance:: Ampicillin

Length:: 3716 bp

Type:: pET & Duet Vectors (Novagen)

Replication origin:: ori

Source/Author:: Novagen (EMD Millipore)

Copy Number:: High copy number

pET-20b(+) vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pET-20b(+) vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pET-20b(+).        3716 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector that encodes a signal sequence for expression of 
            proteins in the periplasm.
ACCESSION   .
VERSION     .
KEYWORDS    pET-20b(+).
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(140..157)
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    158..225
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     sig_peptide     complement(224..289)
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     RBS             complement(297..319)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(351..369)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             878..1066
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    1171..1313
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1499..2087)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2261..3118)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3119..3223)
                     /label=AmpR promoter
     rep_origin      complement(3250..3705)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"