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pET-17b vector (V011027)

Basic Vector Information

Vector Name:
pET-17b
Antibiotic Resistance:
Ampicillin
Length:
3306 bp
Type:
pET & Duet Vectors (Novagen)
Replication origin:
ori
Source/Author:
Novagen (EMD Millipore)
Copy Number:
High copy number

pET-17b vector Vector Map

pET-17b3306 bp6001200180024003000T7 terminatorMCST7 tag (gene 10 leader)RBST7 promoterropbomoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pET-17b vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pET-17b.        3306 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expression of N-terminally T7-tagged proteins.
ACCESSION   .
VERSION     .
KEYWORDS    pET-17b.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(28..75)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    141..228
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             complement(237..269)
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     RBS             complement(277..299)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(331..349)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             858..1046
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    1151..1293
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1479..2067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2241..3098)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3099..3203)
                     /label=AmpR promoter

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