pMAL-p5G vector (V012447)

MSDS

Basic Vector Information

- - Vector Name:
  - pMAL-p5G
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 5755 bp
- - Type:
  - Cloning Vectors
- - Replication origin:
  - ori
- - Source/Author:
  - Riggs P.
- - Copy Number:
  - High copy number

pMAL-p5G vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMAL-p5G vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pMAL-p5G.        5755 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for inducible periplasmic expression of 
            maltose-binding protein (MBP) fusions with a Genenase(TM) I cleavage
            site.
ACCESSION   .
VERSION     .
KEYWORDS    pMAL-p5G.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Riggs P.
  TITLE     Expression and purification of maltose-binding protein fusions.
  JOURNAL   Curr Protoc Mol Biol 2001;Chapter 16:Unit16.6.
  PUBMED    18265133
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5755)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol"; date: "2001"; volume: "Chapter 16"; pages: "Unit16.6"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..80
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             81..1160
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1176..1197
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1406..1434
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1442..1458
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1528..2703
                     /label=MBP
                     /note="maltose binding protein from E. coli"
     CDS             2752..2769
                     /label=Genenase(TM) I site
                     /note="recognition and cleavage site for Genenase(TM) I, a 
                     subtilisin BPN' variant engineered for increased substrate 
                     specificity (Carter and Wells, 1987)"
     misc_feature    2767..2840
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      2841..2927
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3019..3046
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        3073..3164
                     /label=AmpR promoter
     CDS             3165..4022
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4113..4701
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5074..5262)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"

This page is informational only.