Basic Vector Information
pGS-21a vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pGS-21a vector Sequence
LOCUS pGS-21a. 6169 bp DNA circular SYN 01-JAN-1980
DEFINITION Bacterial vector for expression of 6xHis- and GST-tagged proteins.
Two 6xHis sequences are encoded in the vector.
ACCESSION .
VERSION .
KEYWORDS pGS-21a.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6169)
AUTHORS GenScript
TITLE Direct Submission
REFERENCE 2 (bases 1 to 6169)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6169
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..19
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 20..44
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 59..81
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 89..91
/codon_start=1
/product="start codon"
/label=start codon
/note="ATG"
/translation="M"
CDS 101..118
/label=6xHis
/note="6xHis affinity tag"
CDS 128..781
/label=GST
/note="glutathione S-transferase from Schistosoma
japonicum"
CDS 818..832
/label=enterokinase site
/note="enterokinase recognition and cleavage site"
misc_feature 834..892
/label=MCS
/note="MCS"
/note="multiple cloning site"
CDS 893..910
/label=6xHis
/note="6xHis affinity tag"
terminator 977..1024
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 1061..1516
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1543..1647
/label=AmpR promoter
CDS 1648..2505
/label=AmpR
/note="beta-lactamase"
rep_origin 2679..3267
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(3453..3595)
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(3700..3888)
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
protein_bind complement(4663..4684)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(4700..5779)
/label=lacI
/note="lac repressor"
promoter complement(5780..5857)
/label=lacI promoter
This page is informational only.