Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V011649 | pFastBac Dual | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
pFastBac Dual vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pFastBac Dual vector Sequence
LOCUS pFastBac_Dual. 5238 bp DNA circular SYN 01-JAN-1980
DEFINITION Dual-promoter vector for co-expression of two proteins in insect
cells using the Bac-to-Bac(R) baculovirus system.
ACCESSION .
VERSION .
KEYWORDS pFastBac Dual.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5238)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5238)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT Transform into DH10Bac(TM) E. coli cells.
FEATURES Location/Qualifiers
source 1..5238
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 102..557
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 584..688
/label=AmpR promoter
CDS 689..1546
/label=AmpR
/note="beta-lactamase"
rep_origin 1720..2308
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
mobile_element complement(2611..2835)
/label=Tn7R
/note="mini-Tn7 element (right end of the Tn7 transposon)"
CDS complement(2905..3435)
/label=GmR
/note="gentamycin acetyltransferase"
promoter complement(3624..3652)
/label=Pc promoter
/note="class 1 integron promoter"
polyA_signal complement(4161..4209)
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
misc_feature 4274..4337
/label=MCS
/note="MCS"
/note="multiple cloning site"
promoter complement(4345..4454)
/label=p10 promoter
/note="baculovirus promoter for expression in insect cells"
promoter 4478..4569
/label=polyhedrin promoter
/note="promoter for the baculovirus polyhedrin gene"
misc_feature 4606..4704
/label=MCS
/note="MCS"
/note="multiple cloning site"
polyA_signal 4828..4962
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
mobile_element complement(4991..5156)
/label=Tn7L
/note="mini-Tn7 element (left end of the Tn7 transposon)"