pFastBac1 vector (V011648)

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V011648	pFastBac1	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

Vector Name:: pFastBac1

Antibiotic Resistance:: Ampicillin

Length:: 4776 bp

Type:: Insect Cell Vectors

Replication origin:: ori

Source/Author:: Invitrogen (Life Technologies)

Copy Number:: High copy number

Promoter:: polyhedrin

pFastBac1 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pFastBac1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pFastBac1.        4776 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for high-level expression of proteins in insect cells using 
            the Bac-to-Bac(R) baculovirus system.
ACCESSION   .
VERSION     .
KEYWORDS    pFastBac1.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Transform into DH10Bac(TM) E. coli cells.
FEATURES             Location/Qualifiers
     source          1..4776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /label=AmpR promoter
     CDS             589..1446
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1620..2208
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(2511..2735)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2805..3335)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(3524..3552)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    4032..4142
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    4266..4400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(4429..4594)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"