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How to confirm an insert in a plasmid without sequencing?

If you cloned your gene via restriction sites, then you can cleave your ligated vector with the respective enzymes and release your insert. This insert should have the correct size. Additionally, you can cleave with two restriction enzymes, one ...

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Why is enzymatic activity usually pH dependent?

The pH dependence is usually due to the side groups of the amino acids. A change in pH changes the protonation pattern and can, in extreme cases, result in protein denaturation. I therefore suspect that your protein stabilizes the enzyme structure, t...

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Nature: Empathy could be genetic related

Introduction: Empathy is also known as sympathy, etc. It refers to the ability to sense other people's feelings. It has been interpreted in the field of psychology. But recently, scientists at the University of Cambridge in the United Kingdom hav...

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When and why do we need data normalization?

We do data normalization when seeking for relations. Some people do this methods, unfortunately, in experimental designs, which is not correct except if the variable is a transformed one, and all the data needs the same normalization method, such as ...

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Why should we add FBS to the serum free media?

Tissue cells are inherently suicidal and they require growth factors that not only stimulate replication but are generally required to tell the cell not to undergo apoptosis. Remember that most cells (differentiated or not) know were they are because...

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