There are several differences. But first, you have to differentiate between different experiments with different objectives in different species.a) Mice are not man: Standard anti-mouse anti-CD3 antibody (hamster-anti-mouse 145-2C11) has intermediate...
View MoreThere are some articles on this matter:= Formaldehyde (Formalin) fixation at lower temperature (4°C) better preserves Nucleic acid integrity (2011), cf.( http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0021043)= Formaldehyde (Forma...
View MoreIntroduction: For a long time, scientists believe that every day, the human brain's hippocampus will produce hundreds of new nerve cells. However, one of the latest results published in Nature may have to subvert this idea. The new study found th...
View MoreSDS-PAGE points to consider.1. NEVER take an SDS-PAGE for face-value! People do this all to often. If you see one 50kDa band, and nothing else, great, you've got protein >95% pure. If you see multiple bands, you may have your protein really pu...
View MorePHA and PMA/Iono act on cells in a quite different manner.PHA (Phytohaemaglutinin) is a lectin, binds to the sugars on glycosylated surface proteins, including the TCR, and thereby crosslinks them. So with PHA you get TCR crosslinking and signal 1 (a...
View MoreFor biochemistry, a “polymorphism” is one of multiple alternative forms within a protein or nucleic acid sequence. For genetics, a polymorphism refers to genetic variants within the population that allow evolution by natural selection. A “mutati...
View MoreScientists seized the evidence of intestinal bacteria "doing evil" in another Science paper published on the same day!Science（DOI: 10.1126/science.aar7201）In recent years, more and more research shows that the intestinal bacteria living in ...
View MoreA few days ago, an article published in the journal Science revealed that the estimated extent of gene life expectancy accounts for only 16%. This means that the impact of the environment on life expectancy is higher than our expectations. Now, Natur...
View MoreThe term "homology" pertains to comparative studies. Homology indicates an ancient common origin and temporal evolution and refers to structural characteristics. In comparative anatomy, it is used to compare structures in different an...
View MoreBacteria contamination will release LPS in media. LPS, which is a bacterial toxin will strongly change the cellular metabolism that will lead to a false cell response in you experimental model. So, the best option is to discard the cell.You need to c...
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