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Why is enzymatic activity usually pH dependent?

The pH dependence is usually due to the side groups of the amino acids. A change in pH changes the protonation pattern and can, in extreme cases, result in protein denaturation. I therefore suspect that your protein stabilizes the enzyme structure, t...

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Nature: Empathy could be genetic related

Introduction: Empathy is also known as sympathy, etc. It refers to the ability to sense other people's feelings. It has been interpreted in the field of psychology. But recently, scientists at the University of Cambridge in the United Kingdom hav...

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When and why do we need data normalization?

We do data normalization when seeking for relations. Some people do this methods, unfortunately, in experimental designs, which is not correct except if the variable is a transformed one, and all the data needs the same normalization method, such as ...

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Why should we add FBS to the serum free media?

Tissue cells are inherently suicidal and they require growth factors that not only stimulate replication but are generally required to tell the cell not to undergo apoptosis. Remember that most cells (differentiated or not) know were they are because...

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Suggestions of His-tag protein Ni-NTA purification

SDS-PAGE points to consider.1. NEVER take an SDS-PAGE for face-value! People do this all to often. If you see one 50kDa band, and nothing else, great, you've got protein >95% pure. If you see multiple bands, you may have your protein really pu...

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