The pH dependence is usually due to the side groups of the amino acids. A change in pH changes the protonation pattern and can, in extreme cases, result in protein denaturation. I therefore suspect that your protein stabilizes the enzyme structure, t...
View MoreIntroduction: Empathy is also known as sympathy, etc. It refers to the ability to sense other people's feelings. It has been interpreted in the field of psychology. But recently, scientists at the University of Cambridge in the United Kingdom hav...
View MoreWe do data normalization when seeking for relations. Some people do this methods, unfortunately, in experimental designs, which is not correct except if the variable is a transformed one, and all the data needs the same normalization method, such as ...
View MoreTissue cells are inherently suicidal and they require growth factors that not only stimulate replication but are generally required to tell the cell not to undergo apoptosis. Remember that most cells (differentiated or not) know were they are because...
View MoreThere are several differences. But first, you have to differentiate between different experiments with different objectives in different species.a) Mice are not man: Standard anti-mouse anti-CD3 antibody (hamster-anti-mouse 145-2C11) has intermediate...
View MoreThere are some articles on this matter:= Formaldehyde (Formalin) fixation at lower temperature (4°C) better preserves Nucleic acid integrity (2011), cf.( http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0021043)= Formaldehyde (Forma...
View MoreIntroduction: For a long time, scientists believe that every day, the human brain's hippocampus will produce hundreds of new nerve cells. However, one of the latest results published in Nature may have to subvert this idea. The new study found th...
View MoreSDS-PAGE points to consider.1. NEVER take an SDS-PAGE for face-value! People do this all to often. If you see one 50kDa band, and nothing else, great, you've got protein >95% pure. If you see multiple bands, you may have your protein really pu...
View MorePHA and PMA/Iono act on cells in a quite different manner.PHA (Phytohaemaglutinin) is a lectin, binds to the sugars on glycosylated surface proteins, including the TCR, and thereby crosslinks them. So with PHA you get TCR crosslinking and signal 1 (a...
View MoreFor biochemistry, a “polymorphism” is one of multiple alternative forms within a protein or nucleic acid sequence. For genetics, a polymorphism refers to genetic variants within the population that allow evolution by natural selection. A “mutati...
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