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SUMO Protease Cleavage Site

SUMO Protease, also known as Ulp, is a recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae. It is highly specific for the SUMO protein fusion, recognizing the tertiary structure of SUMO rather than an amin...

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Adeno-associated virus (AAV) packing and plasmids

Adeno-associated virus (AAV) is a replication-defective single-stranded DNA virus of the Parvoviridae family. Productive infection of AAV requires the presence of a helper virus, such as adenovirus (Ad) or herpes simplex virus(HSV). AAV has a g...

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Terrific Broth (TB) preparation

Terrific Broth (TB)12 g/L tryptone (1.2% m/V)24 g/L yeast extract (2.4% m/V)4 mL/L glycerol (4.34 mM, 0.4% V/V)17 mM KH2PO4, 2.31 g/L72 mM K2HPO4, 12.54 g/L(opt.) 15 g/L agar (1.5% m/V)(opt., nontrad.) 5–10 mM 1 M MgSO4  or MgCl2The original sug...

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mScarlet3 Protein: A New Tool for Imaging Living Cells

mScarlet3 is a type of red fluorescent protein that has been developed by researchers to label specific structures and organelles in cells. It is a monomeric protein, which means that it exists as a single molecule rather than forming aggregates. Th...

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