Mouse CD95/APO-1/TNFRSF6 (His Tag) recombinant protein

Mouse CD95/APO-1/TNFRSF6 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity).

Tumor necrosis factor receptor superfamily member 6

AI196731; TNFR6; ALPS1A; FAS; APO-1; FASTM; FAS1; APT1; LPR; APO1; CD95; TNFRSF6

P25446

Fas; Apt1; Tnfrsf6

Human Cells

A DNA sequence encoding the extracellular domain of mouse FAS (NP_032013.2) (Met 1-Arg 169) was fused with a polyhistidine tag at the C-terminus.

Mouse

The recombinant mouse FAS consists of 159 amino acids and has a predicted molecular mass of 18 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFAS is approximately 30-35 kDa due to glycosylation.

> 98 % as determined by SDS-PAGE

Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells.
The ED₅₀ for this effect is typically 1-8 μg/mL in the presence of 20 ng/mL recombinant human Fas ligand.