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FAS/CD95 antibody

Cat.#: 107204

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Special Price 239.0 USD

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Product Information

  • Product Name
    FAS/CD95 antibody
  • Documents
  • Description
    FAS/CD95 Mouse Monoclonal antibody. Positive FC detected in Jurkat cells. Positive WB detected in MCF7 cells. Observed molecular weight by Western-blot: 38 kDa
  • Tested applications
    ELISA, WB, FC
  • Species reactivity
    Human,Mouse; other species not tested.
  • Alternative names
    ALPS1A antibody; APO 1 antibody; Apo 1 antigen antibody; APT1 antibody; CD95 antibody; FAS antibody; FAS1 antibody; FASLG receptor antibody; FASTM antibody; TNFRSF6 antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was obtained by immunization of FAS/CD95 recombinant protein (Accession Number: NM_000043). Purification method: Protein G purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

  • Validations

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:107204(FAS antibody) at dilution of 1:500

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:107204(FAS antibody) at dilution of 1:500

    1X10^6 Jurkat cells were stained with 0.2ug FAS antibody (Catalog No:107204, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

    1X10^6 Jurkat cells were stained with 0.2ug FAS antibody (Catalog No:107204, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

  • Background
    FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediates apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). This anti-Fas monoclonal antibody can be used to induce apoptosis in cell cultures through Fas by imitating the Fas-ligand.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"