Rhesus EphB1/Eph Receptor B1 (Fc Tag) recombinant protein

Rhesus EphB1/Eph Receptor B1 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Receptor protein-tyrosine kinase

HEK6; 9330129L11; EPHB1; ENSMUSG00000074119; NET; ELKH; ELK; C130099E04RIK; FLJ37986; EPHT2; CEK6; AW488255

F7DM27

LOC720099; EGK_12084

Human Cells

A DNA sequence encoding the rhesus EPHB1 (XP_001115263.1) (Met1-Pro540) was expressed with the Fc region of human IgG1 at the C-terminus.

Rhesus

The recombinant rhesus EPHB1 comprises 764 amino acids and has a calculated molecular mass of 85.4 KDa.

> 85 % as determined by SDS-PAGE

Immobilized human EFNB1-His (Cat:501410 at 10 μg/ml (100 μl/well) can bind Rhesus EPHB1-Fc, The EC₅₀ of Rhesus EPHB1-Fc is 24.5-57.3 ng/ml.