Mouse EphB1/Eph Receptor B1 (His & GST Tag) recombinant protein

Cat.#: 503163

Size:

Special Price 442.8 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Mouse EphB1/Eph Receptor B1 (His & GST Tag) recombinant protein
  • Documents
  • Description
    Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.
  • Protein name
    Ephrin type-B receptor 1
  • Protein short names
    AW488255; CEK6; EPHT2; C130099E04RIK; ELKH; ELK; NET; FLJ37986; EPHB1; 9330129L11; HEK6; ENSMUSG00000074119
  • Uniprot ID
    Q8CBF3
  • Gene Name
    Ephb1
  • Source/Expression Host
    Baculovirus-Insect Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the cytoplasmic domain (Met 591-Ala 984) of mouse EPHB1 (Q8CBF3-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
  • Protein Species
    Mouse
  • Molecular weight
    The secreted recombinant mouse EPHB1/GST chimera consists of 631 amino acids and has a calculated molecular mass of 72.4 kDa. The recombinant protein migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
  • Purity
    > 95 % as determined by SDS-PAGE
  • Activity
    The specific activity was determined to be 234 nmol/min/mg using Poly(Glu,Tyr) 4:1 as substrate.
  • Validations

    Mouse EphB1 / EPHT2 Protein (His & GST Tag) SDS-PAGE

    Mouse EphB1 / EPHT2 Protein (His & GST Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"