Mouse EphB1/Eph Receptor B1 (His Tag) recombinant protein

Mouse EphB1/Eph Receptor B1 (His Tag) recombinant protein

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Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively.

Ephrin type-B receptor 1

AW488255; EPHT2; CEK6; FLJ37986; NET; C130099E04RIK; ELKH; ELK; ENSMUSG00000074119; EPHB1; 9330129L11; HEK6

Q8CBF3

Ephb1

Human Cells

A DNA sequence encoding the mouse EPHB1 (Q8CBF3-1) extracellular domain (Met 1-Leu 539) was expressed, with a polyhistidine tag at the C-terminus.

Mouse

The secreted recombinant mouse EPHB1 consists of 533 amino acids and has a predicted molecular mass of 59.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm EPHB1 is approximately 64 kDa due to glycosylation.

> 98 % as determined by SDS-PAGE

Immobilized mouse EPHB1-His at 10 μg/ml (100 μl/well) can bind mouse EFNB1-Fc (Cat:503291), The EC₅₀ of mouse EFNB1-Fc (Cat:503291) is 0.04-0.08 μg/ml.