Human HER3/ErbB3 (His Tag) recombinant protein

Human HER3/ErbB3 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

Receptor tyrosine-protein kinase erbB-3

C76256; ERBB3-S; LCCS2; MGC88033; ERBB3R; HER3; P180-ERBB3; MDA-BF-1; C-ERBB3; C-ERBB-3; ERBB-3; ERBB3; P45-SERBB3; MGC117742; P85-SERBB3

P21860

ERBB3; HER3

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Thr 643) of human ErbB3 (NP_001973.2) precursor was expressed, with a C-terminal polyhistidine tag.

Human

The recombinant human ErbB3 protein consists of 635 amino acids after the removal of signal peptide and has a calculated molecular mass of 70.2 kDa. As a result of glycosylation, the recombinant ErbB3 migrates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized human ErbB3 at 2 μg/mL (100 μl/well) can bind human NRG1 (isoform Beta1), The EC₅₀ of human NRG1 (isoform Beta1) is 0.43 μg/mL.