Human HER3/ErbB3 (aa 730-1065, His & GST Tag) recombinant protein

Human HER3/ErbB3 (aa 730-1065, His & GST Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

Receptor tyrosine-protein kinase erbB-3

P21860

ERBB3; HER3

Baculovirus-Insect Cells

A DNA sequence encoding the cytoplasmic domain (Pro 730-Ser 1065) of human ErbB3 (NP_001973.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human ErbB3 /GST chimera consists of 573 amino acids and has a calculated molecular mass of 65 KDa. It migrates as an approximately 65 KDa band as predicted in SDS-PAGE under reducing conditions.

> 82 % as determined by SDS-PAGE

1. No Kinase Activity
2. Using the Octet RED System, the affinity constant (Kd) of human ErBB3-GST (Cat:503150) bound to human NRG1 (aa 2-224) (Cat:504102) was 20 nM.