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Product Name
Anti-ASS1 (6C8) Mouse antibody
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Description
ASS1 (6C8) Mouse monoclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Monkey
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human ASS1 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ASS1 mouse mAb with DAB staining.
Western blot detection of ASS1 in C6,Jurkat,K562,3T3 and Hela cell lysates using ASS1 mouse mAb (1:3000 diluted).Predicted band size:47KDa.Observed band size:47KDa.
Western blot analysis using ASS1 mouse mAb against A431 (1), RAJI (2), MOLT4 (3), Jurkat (4), A549 (5), NIH/3T3 (6), PC-12 (7) and Cos7 (8) cell lysate.
Immunohistochemical analysis of paraffin-embedded colon cancer tissues using ASS1 mouse mAb with DAB staining.
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Background
Swiss-Prot Acc.P00966.The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"