-
Product Name
ASS1 antibody
- Documents
-
Description
ASS1 Mouse Monoclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human liver tissue, human testis tissue. Positive WB detected in Hela cells, human liver tissue. Observed molecular weight by Western-blot: 40-45kD
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human; other species not tested.
-
Alternative names
Argininosuccinate synthase antibody; argininosuccinate synthetase 1 antibody; ASS antibody; ASS1 antibody; Citrulline aspartate ligase antibody; CTLN1 antibody
-
Isotype
Mouse IgG1
-
Preparation
This antibody was obtained by immunization of ASS1 recombinant protein (Accession Number: NM_054012). Purification method: Protein G purified.
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107068(ASS1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:107068(ASS1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107068(ASS1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using ASS1 antibody Catalog No: at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
-
Background
ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).
-
References
- Liu J, Ma J, Wu Z. Arginine deiminase augments the chemosensitivity of argininosuccinate synthetase-deficient pancreatic cancer cells to gemcitabine via inhibition of NF-κB signaling. BMC cancer. 14:686. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"