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Product Name
Anti-ASS1 (2C8) Mouse antibody
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Description
ASS1 (2C8) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat, Monkey
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human ASS1 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Flow cytometric analysis of Jurkat cells using ASS1 mouse mAb (green) and negative control (red).
Western blot analysis of extracts from A9,THP-1,C2C12 and 3T3 cell lysates using ASS1 mouse mAb (1:1000 diluted).Predicted band size:47KDa.Observed band size:47KDa.
Western blot analysis using ASS1 mouse mAb against A431 (1), RAJI (2), L1210 (3), MOLT4 (4), Jurkat (5), A549 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
Immunofluorescence analysis of Hela cells using ASS1 mouse mAb (green). Blue
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Background
Swiss-Prot Acc.P00966.The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"