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Product Name
Mouse ALK-1 / ACVRL1 (His & Fc Tag) recombinant protein
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Description
Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.
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Protein name
Serine/threonine-protein kinase receptor R3
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Protein short names
ORW2; ALK1; R3; SKR3; HHT; ALK-1; HHT2; SETHKIR; ACVRLK1; MGC91691; R-3; AI427544; AI115505; ACVRL1; TSR-I
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Uniprot ID
Q61288
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Gene Name
Acvrl1; Acvrlk1; Alk-1
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse ALK1 (NP_033742.2) precursor (Met 1-Pro 119) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The secreted recombinant mouse ALK1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer comprises 345 amino acids with a predicted molecular mass of 39 kDa. As a result of glycosylation, it migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its ability to inhibit BMP9 induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953.
The ED50 for this effect is typically 60-300 ng/mL in the presence of 2 ng/mL of recombiant human BMP9. -
Validations
Mouse ALK-1 / ACVRL1 Protein (His & Fc Tag) SDS-PAGE
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