Human PCSK9 (His Tag) recombinant protein

Human PCSK9 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.

Proprotein convertase subtilisin/kexin type 9

PSEC0052; MACACA MULATTA; LDLCQ1; AI747682; AI415265; MGC47409; PCSK9; PC9; FH3; NARC-1; HCHOLA3; NARC1; RP23-159J7.1

Q8NBP7

PCSK9; NARC1; PSEC0052

Human Cells

A DNA sequence encoding the human proprotein convertase 9 (AAV67948.1) (Met 1-Gln 692) was expressed with a C-terminal polyhistidine tag.

Human

The recombinant human PCSK9 consists of 662 amino acids and predicts a molecular mass of 72.6 kDa. As a result of glycosylation and proteolytic digestion, rhPCSK9 migrates as doublet with apparent molecular mass of 20 kDa and 62 kDa respectively in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

Measure by its ability to bind with human LDLR in a functional ELISA. Immobilized recombinant human PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated human LDLR. The EC₅₀ of biotinylated human LDLR is 0.61 μg/ml.