Rat PCSK9 (His Tag) recombinant protein

Rat PCSK9 (His Tag) recombinant protein

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• Material Safety Data Sheets (MSDS)

Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways (By similarity).

Proprotein convertase subtilisin/kexin type 9

LDLCQ1; PSEC0052; MACACA MULATTA; AI747682; AI415265; FH3; NARC-1; MGC47409; PC9; PCSK9; RP23-159J7.1; HCHOLA3; NARC1

P59996

Pcsk9; Narc1

Human Cells

A DNA sequence encoding the rat PCSK9 (NP_954862.2) (Met 1-Gln 691) was expressed, fused with a polyhistidine tag at the C-terminus.

Rat

The secreted recombinant pro form of rat PCSK9 comprises 672 amino acids and predicts a molecular mass of 72.8 kDa. As a result of glycosylation and proteolytic digestion, rat PCSK9 migrates as doublet with apparent molecular mass of 20 kDa and 62 kDa corresponding to the pro domain and mature form respectively in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

1. Measured by its ability to bind biotinylated human LDLR in a functional ELISA.
2. Measured by its ability to bind biotinylated mouse LDLR in a functional ELISA.