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Product Name
MPO antibody
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Description
MPO Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, MCF7 cells, U-937 cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 59 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
MPO antibody; myeloperoxidase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MPO recombinant protein (Accession Number: NM_000250). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112746(MPO antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human liver using Catalog No:112746(MPO antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:112746(MPO antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-MPO (IP:Catalog No:112746, 4ug; Detection:Catalog No:112746 1:600) with HeLa cells lysate 1600ug.
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Background
The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing. This antibody is specific to MPO.
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