Mouse TRAIL R2/CD262/TNFRSF10B (His & Fc Tag) recombinant protein

Mouse TRAIL R2/CD262/TNFRSF10B (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.

Tumor necrosis factor receptor superfamily member 10B

ZTNFR9; KILLER/DR5; CD262; KILLER; TRAIL-R2; TRAILR2; TRICK2A; TRICK2B; DR5; TNFRSF10B; TRAIL R2; MK; TRICKB; TRICK2; LY98

Q9QZM4

Tnfrsf10b; Dr5; Killer

Human Cells

A DNA sequence encoding the extracellular domain of mouse TNFRSF10B (NP_064671.2) (Met 1-Ser 177) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Mouse

The recombinant mouse TNFRSF10B/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 373 amino acids and has a calculated molecular mass of 41.8 kDa. As a result of glycosylation, the apparent molecular mass of the TNFRSF10B/Fc monomer is approximately 50-55 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Immobilized human TNFSF10 (Cat:501935) at 10 μg/ml (100 μl/well) can bind mouse TNFRSF10B-Fch, The EC₅₀ of mouse TNFRSF10B-Fch is 0.07-0.17 μg/ml.
2. Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED₅₀ for this effect is typically 20-80 ng/ml in the presence of 20 ng/ml Recombinant Human TRAIL/TNFSF10.