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Product Name
Kynurenine 3 monooxygenase antibody
- Documents
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Description
Kynurenine 3 monooxygenase Rabbit Polyclonal antibody. Positive WB detected in mouse heart tissue, HeLa cells, mouse spleen tissue, rat spleen tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue, human prostate cancer tissue, mouse heart tissue. Observed molecular weight by Western-blot: 55.8 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
dJ317G22.1 antibody; KMO antibody; Kynurenine 3 hydroxylase antibody; Kynurenine 3 monooxygenase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Kynurenine 3 monooxygenase recombinant protein (Accession Number: BC005297). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:112261(KMO antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:112261(KMO antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:112261(KMO antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:112261(KMO Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-tryptophan metabolite L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).
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References
- Winkler D, Beconi M, Toledo-Sherman LM. Development of LC/MS/MS, high-throughput enzymatic and cellular assays for the characterization of compounds that inhibit kynurenine monooxygenase (KMO). Journal of biomolecular screening. 18(8):879-89. 2013.
- Wang Q, Zhang M, Ding Y. Activation of NAD(P)H oxidase by tryptophan-derived 3-hydroxykynurenine accelerates endothelial apoptosis and dysfunction in vivo. Circulation research. 114(3):480-92. 2014.
- Rojewska E, Piotrowska A, Makuch W, Przewlocka B, Mika J. Pharmacological kynurenine 3-monooxygenase enzyme inhibition significantly reduces neuropathic pain in a rat model. Neuropharmacology. 102:80-91. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"