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Product Name
ITPA antibody
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Description
ITPA Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human heart tissue, human lung cancer tissue. Positive IP detected in mouse liver tissue. Positive WB detected in K-562 cells, HeLa cells, HT-1080 cells, human liver tissue, mouse heart tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C20orf37 antibody; dJ794I6.3 antibody; HLC14 06 P antibody; Inosine triphosphatase antibody; ITPA antibody; ITPase antibody; OK/SW cl.9 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ITPA recombinant protein (Accession Number: NM_033453). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:111955(ITPA antibody) at dilution of 1:1000
Immunofluorescent analysis of MCF-7 cells, using ITPA antibody Catalog No:109111 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-ITPA (IP:Catalog No:111955, 4ug; Detection:Catalog No:111955 1:1000) with mouse liver tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:111955(ITPA Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:111955(ITPA Antibody) at dilution of 1:50 (under 40x lens)
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Background
ITPA(Inosine triphosphate pyrophosphatase) catalyzes the pyrophosphohydrolysis of inosine triphosphate (ITP) to inosine monophosphate (IMP).It expresses in the cytoplasm(PMID:11278832).It can be detected a band of 44 Kda as a homodimer in the western blot(PMID:19631656) though its predictedsize is 21kd.This protein has two isforms with the molecular weight of 20KDa and 21KDa.
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References
- Herting G, Barber K, Zappala MR, Cunningham RP, Burgis NE. Quantitative in vitro and in vivo characterization of the human P32T mutant ITPase. Biochimica et biophysica acta. 1802(2):269-74. 2010.
- Gall AD, Gall A, Moore AC. Analysis of human ITPase nucleobase specificity by site-directed mutagenesis. Biochimie. 95(9):1711-21. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"