Human EphA4/Eph Receptor A4 (His Tag) recombinant protein

Human EphA4/Eph Receptor A4 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

EPH receptor A4 (ephrin type-A receptor 4), also known as EphA4, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus, and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor−mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). The extracellular domain of an EphA4 interacts with ephrin ligands, which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer.

Ephrin type-A receptor 4

SEK; HEK8; AI385584; 2900005C20RIK; SEK1; TYRO1; CEK8; EPHA4; RB

Q03137

Epha4; Sek; Sek1

Human Cells

A DNA sequence encoding the human EPHA4 (NP_031962.2) extracellular domain (Met 1-Thr 547) was expressed, with a polyhistidine tag at the C-terminus.

Human

The recombinant human EPHA4 consists of 539 amino acids and predictes a molecular mass of 60 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EPHA4 is approximately 60-70 kDa due to high levels of glycosylation.

> 96 % as determined by SDS-PAGE

Measured by its ability to bind biotinylated Human EphrinA5-his (cat: 501087) in functional ELISA.