Human EphA4/Eph Receptor A4 (aa 570-986, His & GST Tag) recombinant protein

Human EphA4/Eph Receptor A4 (aa 570-986, His & GST Tag) recombinant protein

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Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.

Ephrin type-A receptor 4

HEK8; SEK; 2900005C20RIK; AI385584; EPHA4; CEK8; TYRO1; SEK1; RB

P54764

EPHA4; HEK8; SEK; TYRO1

Baculovirus-Insect Cells

A DNA sequence encoding the human EPHA4 (P54764) (Ser570-Val986) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human EPHA4 /GST chimera consists of 654 amino acids and has a calculated molecular mass of 75 kDa. The recombinant protein migrates as an approximately 67 kDa band in SDS-PAGE under reducing conditions.

> 99 % as determined by SDS-PAGE

1. The specific activity was determined to be 17 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
2. Measured by its binding ability in a functional ELISA. Immobilized human EPHA4 (aa 570-986) (Cat: 502527) at 10 μg/ml (100 μl/well) can bind biotinylated human EphrinA5-His with a linear range of 0.625-5.0 μg/ml.