-
Product Name
Human DDR1/MCK10/CD167 (aa 444-913, His & GST Tag) recombinant protein
- Documents
-
Description
Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.
-
Protein name
Epithelial discoidin domain-containing receptor 1
-
Protein short names
NEP; CD167A; TRKE; NTRK4; DDR; AI323681; HGK2; DAAP-278B20.1; CAK; EDDR1; PTK3; 6030432F18; DDR1; MCK10; RTK6; CD167; PTK3A
-
Uniprot ID
Q08345
-
Gene Name
DDR1; CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE
-
Source/Expression Host
Baculovirus-Insect Cells
-
Expression Plasmid/cDNA
A DNA sequence encoding the human DDR1 (Q08345-1) (Arg444-Val913) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
-
Protein Species
Human
-
Molecular weight
The recombinant human DDR1 /GST chimera consists of 707 amino acids and has a calculated molecular mass of 80 kDa. The recombinant protein migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
-
Purity
> 89 % as determined by SDS-PAGE
-
Activity
The specific activity was determined to be 2.75 nmol/min/mg using synthetic AXLtide peptide(CKKSRGDYMTMQIG) as substrate.
-
Validations
Human DDR1 Kinase / MCK10 / CD167 Protein (aa 444-913, His & GST Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"