Human CD122 / IL-2RB (Fc Tag) recombinant protein

Human CD122 / IL-2RB (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.

Interleukin-2 receptor subunit beta

IL15RB; CD122; RP5-1170K4.6; P70-75; IL-2RB; IL-2RBETA; IL-15RBETA; IL2RB; P70; IL-2/15RBETA; IL15RBETA

P14784

IL2RB

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Asp 239) of human IL2 R? (NP_000869.1) precursor was expressed with the C-terminal fused Fc region of human IgG1.

Human

The recombinant human IL2 R?/Fc is a disulfide-linked homodimeric protein generated after removal of the signal peptide. The reduced monomer consists of 451 amino acids and has a predicted molecular mass of 51.6 kDa. As a result of glycosylation, the apparent molecular mass of rh IL2RB/Fc monomer is approximately 60-65 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

1. Measured by its ability to bind biotinylated recombinant rat IL2 in a functional ELISA.
2. Using the Octet RED System, the affinity constant (Kd) of Human IL2RB-Fc (Cat:503194) bound to Human IL2 was 0.8nM.