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Product Name
EEF2 antibody
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Description
EEF2 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HEK-293 cells, HL-60 cells, Jurkat cells, NIH/3T3 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human stomach cancer tissue, human breast cancer tissue. Observed molecular weight by Western-blot: 95kd,100kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
EEF 2 antibody; EEF2 antibody; EF 2 antibody; EF2 antibody; Elongation factor 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EEF2 recombinant protein (Accession Number: NM_001961). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110316(EEF2 antibody) at dilution of 1:500
IP Result of anti-EEF2 (IP:Catalog No:110316, 3ug; Detection:Catalog No:110316 1:500) with HeLa cells lysate 1600ug.
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:110316(EEF2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:110316(EEF2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using EEF2 antibody Catalog No:110316 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
EEF2 represents eukaryotic translation elongation factor 2
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"