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Product Name
Anti-EEF2/Elongation factor 2 (6E12) Mouse antibody
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Description
EEF2/Elongation factor 2 (6E12) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human EEF2 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and kidney cancer tissues (right) using EEF2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded prostate cancer tissues (left) and tonsil tissues (right) using EEF2 mouse mAb with DAB staining.
Immunofluorescence analysis of 3T3-L1 cells using EEF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot detection of eEF2 in Jurkat,K562 and 3T3 cell lysates using eEF2 mouse mAb (1:1000 diluted).Predicted band size:95KDa.Observed band size:95KDa.
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Background
Swiss-Prot Acc.P13639.This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. (provided by RefSeq)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"