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Product Name
Anti-EEF2/Elongation factor 2 (4B3) Mouse antibody
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Description
EEF2/Elongation factor 2 (4B3) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, ChIP
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Species reactivity
Human
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Alternative names
EEF2;Eef2;EF-2;EF2;EF2_HUMAN;Elongation factor 2;Eukaryotic translation elongation factor 2;Polypeptidyl tRNA translocase;SCA26. antibody
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Isotype
IgG2b
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Preparation
Antigen: Purified recombinant human eEF2 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/5000
;ICC: 1/200
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Validations
Western blot detection of eEF2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using eEF2 mouse mAb (1:5000 diluted).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s.
Immunocytochemistry staining of HeLa cells fixed with -20u2103 Methanol and using anti-eEF2 mouse mAb (dilution 1:200).
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Background
Swiss-Prot Acc.P13639.Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"